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psn1 rabbit polyclonal  (Proteintech)


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    Proteintech psn1 rabbit polyclonal
    Summary of antibody dilutions and conditions used in western blot analysis
    Psn1 Rabbit Polyclonal, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+nt5c3/NT5C3+Antibody/pmc11168514-13-0-8
    Average 92 stars, based on 2 article reviews
    psn1 rabbit polyclonal - by Bioz Stars, 2026-09
    92/100 stars

    Images

    1) Product Images from "MicroRNA-502-3p regulates GABAergic synapse function in hippocampal neurons"

    Article Title: MicroRNA-502-3p regulates GABAergic synapse function in hippocampal neurons

    Journal: Neural Regeneration Research

    doi: 10.4103/NRR.NRR-D-23-01064

    Summary of antibody dilutions and conditions used in western blot analysis
    Figure Legend Snippet: Summary of antibody dilutions and conditions used in western blot analysis

    Techniques Used: Western Blot

    Modulation of AD proteins by miR-502-3p. (A) Immunoblot analysis of amyloid precursor protein (APP), tubulin associated unit (Tau), presenilin-1 (PSN1) and presenilin-2 (PSN2) and -actin protein in hippocampal neurons (HT22) cells transfected with miR-502-3p agomiRs and miR-502-3p antagomiRs. The levels of AD proteins were changed in miR-502-3p agomiR and antagomiR transfected cells. (B) Densitometry analysis of APP, Tau, PSN1 and PSN2 blot shows increased protein levels in miR-502-3p agomiR transfected cells compared with scramble control, while the cells treated with miR-502-3p antagomiR shows a reduced level of all AD proteins compared with agomiRs. For statistical analysis, Student’s t -test was used for analyzing the data between three groups of samples such as scramble control, agomiRs, and antagomiRs. Experiments were performed in triplicates. The P -values < 0.05 were considered statistically significant. AD: Alzheimer’s disease; miR: microRNA.
    Figure Legend Snippet: Modulation of AD proteins by miR-502-3p. (A) Immunoblot analysis of amyloid precursor protein (APP), tubulin associated unit (Tau), presenilin-1 (PSN1) and presenilin-2 (PSN2) and -actin protein in hippocampal neurons (HT22) cells transfected with miR-502-3p agomiRs and miR-502-3p antagomiRs. The levels of AD proteins were changed in miR-502-3p agomiR and antagomiR transfected cells. (B) Densitometry analysis of APP, Tau, PSN1 and PSN2 blot shows increased protein levels in miR-502-3p agomiR transfected cells compared with scramble control, while the cells treated with miR-502-3p antagomiR shows a reduced level of all AD proteins compared with agomiRs. For statistical analysis, Student’s t -test was used for analyzing the data between three groups of samples such as scramble control, agomiRs, and antagomiRs. Experiments were performed in triplicates. The P -values < 0.05 were considered statistically significant. AD: Alzheimer’s disease; miR: microRNA.

    Techniques Used: Western Blot, Transfection, Control

    Related Articles

    Western Blot:

    Article Title: Quantitative sequencing using BID-seq uncovers abundant pseudouridines in mammalian mRNA at base resolution
    Article Snippet: .. The following antibodies were used in this study: rabbit monoclonal anti-NDUFS2, clone EPR16266 (abcam, catalog no. ab192022, 1:1,000), mouse monoclonal anti-GAPDH, clone 0411 (Santa Cruz, catalog no. sc-47724, 1:1,000), rabbit polyclonal anti-SELENOF (My BioSource, catalog no. MBS3208942, 1:500), mouse monoclonal anti-UBE2E3, clone OTI7E8 (Novus Biologicals, catalog no. NBP2-03819, 1:500), rabbit polyclonal anti-PPP1R2 (Thermo Fisher Scientific, catalog no. PA5-115787, 1:500), rabbit polyclonal anti-NT5C3 (Proteintech, catalog no. 11393-1-AP, 1:500), rabbit polyclonal anti-SZRD1 (Thermo Fisher Scientific, catalog no. A304-742A, 1:1,000), rabbit polyclonal anti-SNRPD1 (Novus Biologicals, catalog no. NBP2-36427, 1:500), rabbit polyclonal anti-DNAJC19 (Thermo Fisher Scientific, catalog no. PA5-98770, 1:1,000), rabbit polyclonal anti-MAPKAP1 (Proteintech, catalog no. 15463-1-AP, 1:500), rabbit monoclonal anti-CD52, clone EPR3153(2) (abcam, catalog no. ab125071, 1:1,000), rabbit polyclonal anti-A2LD1 (Proteintech, catalog no. 23280-1-AP, 1:500), anti-rabbit IgG, horseradish peroxidase (HRP)-linked antibody (Cell Signaling, catalog no. 7074S WB, 1:2,000), anti-mouse IgG, HRP-linked antibody (Cell Signaling catalog no. 7076S, WB 1:2,000). .. For transient transfection, cells were transfected with casRx (Addgene catalog no. 109053) gRNA construct and pCMV-TRUB1-dCas13D by Lipofectamine 2000 Transfection Reagent (Invitrogen) according to manufacturer’s protocol, or with siRNA by Lipofectamine RNAiMAX Transfection Reagent (Invitrogen) following commercial protocols.

    Article Title: Quantitative sequencing using BID-seq uncovers abundant pseudouridines in mammalian mRNA at base resolution.
    Article Snippet: .. The following antibodies were used in this study: rabbit monoclonal anti-NDUFS2, clone EPR16266 (abcam, catalog no. ab192022, 1:1,000), mouse monoclonal anti-GAPDH, clone 0411 (Santa Cruz, catalog no. sc-47724, 1:1,000), rabbit polyclonal anti-SELENOF (My BioSource, catalog no. MBS3208942, 1:500), mouse monoclonal anti-UBE2E3, clone OTI7E8 (Novus Biologicals, catalog no. NBP2-03819, 1:500), rabbit polyclonal anti-PPP1R2 (Thermo Fisher Scientific, catalog no. PA5-115787, 1:500), rabbit polyclonal anti-NT5C3 (Proteintech, catalog no. 11393-1-AP, 1:500), rabbit polyclonal anti-SZRD1 (Thermo Fisher Scientific, catalog no. A304-742A, 1:1,000), rabbit polyclonal anti-SNRPD1 (Novus Biologicals, catalog no. NBP2-36427, 1:500), rabbit polyclonal anti-DNAJC19 (Thermo Fisher Scientific, catalog no. PA5-98770, 1:1,000), rabbit polyclonal anti-MAPKAP1 (Proteintech, catalog no. 15463-1-AP, 1:500), rabbit monoclonal anti-CD52, clone EPR3153(2) (abcam, catalog no. ab125071, 1:1,000), rabbit polyclonal anti-A2LD1 (Proteintech, catalog no. 23280-1-AP, 1:500), anti-rabbit IgG, horseradish peroxidase (HRP)-linked antibody (Cell Signaling, catalog no. 7074S WB, 1:2,000), anti-mouse IgG, HRP-linked antibody (Cell Signaling catalog no. 7076S, WB 1:2,000). shRNA knockdown and plasmid transfection For transient transfection, cells were transfected with casRx (Addgene catalog no. 109053) gRNA construct and pCMV-TRUB1-dCas13D by Lipofectamine 2000 Transfection Reagent (Invitrogen) according to manufacturer’s protocol, or with siRNA by Lipofectamine RNAiMAX Transfection Reagent (Invitrogen) following commercial protocols. .. For lentivirus production, a lentiviral construct (pLKO-Tet-On for inducible knockdown of pseudouridine synthetases, or green fluorescent protein as a negative control) together with pMD2.G (Addgene catalog no. 12259) and psPAX2 (Addgene catalog no. 12260) were cotransfected into 293TN cells (System Biosciences) as previously described47.

    shRNA:

    Article Title: Quantitative sequencing using BID-seq uncovers abundant pseudouridines in mammalian mRNA at base resolution.
    Article Snippet: .. The following antibodies were used in this study: rabbit monoclonal anti-NDUFS2, clone EPR16266 (abcam, catalog no. ab192022, 1:1,000), mouse monoclonal anti-GAPDH, clone 0411 (Santa Cruz, catalog no. sc-47724, 1:1,000), rabbit polyclonal anti-SELENOF (My BioSource, catalog no. MBS3208942, 1:500), mouse monoclonal anti-UBE2E3, clone OTI7E8 (Novus Biologicals, catalog no. NBP2-03819, 1:500), rabbit polyclonal anti-PPP1R2 (Thermo Fisher Scientific, catalog no. PA5-115787, 1:500), rabbit polyclonal anti-NT5C3 (Proteintech, catalog no. 11393-1-AP, 1:500), rabbit polyclonal anti-SZRD1 (Thermo Fisher Scientific, catalog no. A304-742A, 1:1,000), rabbit polyclonal anti-SNRPD1 (Novus Biologicals, catalog no. NBP2-36427, 1:500), rabbit polyclonal anti-DNAJC19 (Thermo Fisher Scientific, catalog no. PA5-98770, 1:1,000), rabbit polyclonal anti-MAPKAP1 (Proteintech, catalog no. 15463-1-AP, 1:500), rabbit monoclonal anti-CD52, clone EPR3153(2) (abcam, catalog no. ab125071, 1:1,000), rabbit polyclonal anti-A2LD1 (Proteintech, catalog no. 23280-1-AP, 1:500), anti-rabbit IgG, horseradish peroxidase (HRP)-linked antibody (Cell Signaling, catalog no. 7074S WB, 1:2,000), anti-mouse IgG, HRP-linked antibody (Cell Signaling catalog no. 7076S, WB 1:2,000). shRNA knockdown and plasmid transfection For transient transfection, cells were transfected with casRx (Addgene catalog no. 109053) gRNA construct and pCMV-TRUB1-dCas13D by Lipofectamine 2000 Transfection Reagent (Invitrogen) according to manufacturer’s protocol, or with siRNA by Lipofectamine RNAiMAX Transfection Reagent (Invitrogen) following commercial protocols. .. For lentivirus production, a lentiviral construct (pLKO-Tet-On for inducible knockdown of pseudouridine synthetases, or green fluorescent protein as a negative control) together with pMD2.G (Addgene catalog no. 12259) and psPAX2 (Addgene catalog no. 12260) were cotransfected into 293TN cells (System Biosciences) as previously described47.

    Knockdown:

    Article Title: Quantitative sequencing using BID-seq uncovers abundant pseudouridines in mammalian mRNA at base resolution.
    Article Snippet: .. The following antibodies were used in this study: rabbit monoclonal anti-NDUFS2, clone EPR16266 (abcam, catalog no. ab192022, 1:1,000), mouse monoclonal anti-GAPDH, clone 0411 (Santa Cruz, catalog no. sc-47724, 1:1,000), rabbit polyclonal anti-SELENOF (My BioSource, catalog no. MBS3208942, 1:500), mouse monoclonal anti-UBE2E3, clone OTI7E8 (Novus Biologicals, catalog no. NBP2-03819, 1:500), rabbit polyclonal anti-PPP1R2 (Thermo Fisher Scientific, catalog no. PA5-115787, 1:500), rabbit polyclonal anti-NT5C3 (Proteintech, catalog no. 11393-1-AP, 1:500), rabbit polyclonal anti-SZRD1 (Thermo Fisher Scientific, catalog no. A304-742A, 1:1,000), rabbit polyclonal anti-SNRPD1 (Novus Biologicals, catalog no. NBP2-36427, 1:500), rabbit polyclonal anti-DNAJC19 (Thermo Fisher Scientific, catalog no. PA5-98770, 1:1,000), rabbit polyclonal anti-MAPKAP1 (Proteintech, catalog no. 15463-1-AP, 1:500), rabbit monoclonal anti-CD52, clone EPR3153(2) (abcam, catalog no. ab125071, 1:1,000), rabbit polyclonal anti-A2LD1 (Proteintech, catalog no. 23280-1-AP, 1:500), anti-rabbit IgG, horseradish peroxidase (HRP)-linked antibody (Cell Signaling, catalog no. 7074S WB, 1:2,000), anti-mouse IgG, HRP-linked antibody (Cell Signaling catalog no. 7076S, WB 1:2,000). shRNA knockdown and plasmid transfection For transient transfection, cells were transfected with casRx (Addgene catalog no. 109053) gRNA construct and pCMV-TRUB1-dCas13D by Lipofectamine 2000 Transfection Reagent (Invitrogen) according to manufacturer’s protocol, or with siRNA by Lipofectamine RNAiMAX Transfection Reagent (Invitrogen) following commercial protocols. .. For lentivirus production, a lentiviral construct (pLKO-Tet-On for inducible knockdown of pseudouridine synthetases, or green fluorescent protein as a negative control) together with pMD2.G (Addgene catalog no. 12259) and psPAX2 (Addgene catalog no. 12260) were cotransfected into 293TN cells (System Biosciences) as previously described47.

    Plasmid Preparation:

    Article Title: Quantitative sequencing using BID-seq uncovers abundant pseudouridines in mammalian mRNA at base resolution.
    Article Snippet: .. The following antibodies were used in this study: rabbit monoclonal anti-NDUFS2, clone EPR16266 (abcam, catalog no. ab192022, 1:1,000), mouse monoclonal anti-GAPDH, clone 0411 (Santa Cruz, catalog no. sc-47724, 1:1,000), rabbit polyclonal anti-SELENOF (My BioSource, catalog no. MBS3208942, 1:500), mouse monoclonal anti-UBE2E3, clone OTI7E8 (Novus Biologicals, catalog no. NBP2-03819, 1:500), rabbit polyclonal anti-PPP1R2 (Thermo Fisher Scientific, catalog no. PA5-115787, 1:500), rabbit polyclonal anti-NT5C3 (Proteintech, catalog no. 11393-1-AP, 1:500), rabbit polyclonal anti-SZRD1 (Thermo Fisher Scientific, catalog no. A304-742A, 1:1,000), rabbit polyclonal anti-SNRPD1 (Novus Biologicals, catalog no. NBP2-36427, 1:500), rabbit polyclonal anti-DNAJC19 (Thermo Fisher Scientific, catalog no. PA5-98770, 1:1,000), rabbit polyclonal anti-MAPKAP1 (Proteintech, catalog no. 15463-1-AP, 1:500), rabbit monoclonal anti-CD52, clone EPR3153(2) (abcam, catalog no. ab125071, 1:1,000), rabbit polyclonal anti-A2LD1 (Proteintech, catalog no. 23280-1-AP, 1:500), anti-rabbit IgG, horseradish peroxidase (HRP)-linked antibody (Cell Signaling, catalog no. 7074S WB, 1:2,000), anti-mouse IgG, HRP-linked antibody (Cell Signaling catalog no. 7076S, WB 1:2,000). shRNA knockdown and plasmid transfection For transient transfection, cells were transfected with casRx (Addgene catalog no. 109053) gRNA construct and pCMV-TRUB1-dCas13D by Lipofectamine 2000 Transfection Reagent (Invitrogen) according to manufacturer’s protocol, or with siRNA by Lipofectamine RNAiMAX Transfection Reagent (Invitrogen) following commercial protocols. .. For lentivirus production, a lentiviral construct (pLKO-Tet-On for inducible knockdown of pseudouridine synthetases, or green fluorescent protein as a negative control) together with pMD2.G (Addgene catalog no. 12259) and psPAX2 (Addgene catalog no. 12260) were cotransfected into 293TN cells (System Biosciences) as previously described47.

    Transfection:

    Article Title: Quantitative sequencing using BID-seq uncovers abundant pseudouridines in mammalian mRNA at base resolution.
    Article Snippet: .. The following antibodies were used in this study: rabbit monoclonal anti-NDUFS2, clone EPR16266 (abcam, catalog no. ab192022, 1:1,000), mouse monoclonal anti-GAPDH, clone 0411 (Santa Cruz, catalog no. sc-47724, 1:1,000), rabbit polyclonal anti-SELENOF (My BioSource, catalog no. MBS3208942, 1:500), mouse monoclonal anti-UBE2E3, clone OTI7E8 (Novus Biologicals, catalog no. NBP2-03819, 1:500), rabbit polyclonal anti-PPP1R2 (Thermo Fisher Scientific, catalog no. PA5-115787, 1:500), rabbit polyclonal anti-NT5C3 (Proteintech, catalog no. 11393-1-AP, 1:500), rabbit polyclonal anti-SZRD1 (Thermo Fisher Scientific, catalog no. A304-742A, 1:1,000), rabbit polyclonal anti-SNRPD1 (Novus Biologicals, catalog no. NBP2-36427, 1:500), rabbit polyclonal anti-DNAJC19 (Thermo Fisher Scientific, catalog no. PA5-98770, 1:1,000), rabbit polyclonal anti-MAPKAP1 (Proteintech, catalog no. 15463-1-AP, 1:500), rabbit monoclonal anti-CD52, clone EPR3153(2) (abcam, catalog no. ab125071, 1:1,000), rabbit polyclonal anti-A2LD1 (Proteintech, catalog no. 23280-1-AP, 1:500), anti-rabbit IgG, horseradish peroxidase (HRP)-linked antibody (Cell Signaling, catalog no. 7074S WB, 1:2,000), anti-mouse IgG, HRP-linked antibody (Cell Signaling catalog no. 7076S, WB 1:2,000). shRNA knockdown and plasmid transfection For transient transfection, cells were transfected with casRx (Addgene catalog no. 109053) gRNA construct and pCMV-TRUB1-dCas13D by Lipofectamine 2000 Transfection Reagent (Invitrogen) according to manufacturer’s protocol, or with siRNA by Lipofectamine RNAiMAX Transfection Reagent (Invitrogen) following commercial protocols. .. For lentivirus production, a lentiviral construct (pLKO-Tet-On for inducible knockdown of pseudouridine synthetases, or green fluorescent protein as a negative control) together with pMD2.G (Addgene catalog no. 12259) and psPAX2 (Addgene catalog no. 12260) were cotransfected into 293TN cells (System Biosciences) as previously described47.

    Construct:

    Article Title: Quantitative sequencing using BID-seq uncovers abundant pseudouridines in mammalian mRNA at base resolution.
    Article Snippet: .. The following antibodies were used in this study: rabbit monoclonal anti-NDUFS2, clone EPR16266 (abcam, catalog no. ab192022, 1:1,000), mouse monoclonal anti-GAPDH, clone 0411 (Santa Cruz, catalog no. sc-47724, 1:1,000), rabbit polyclonal anti-SELENOF (My BioSource, catalog no. MBS3208942, 1:500), mouse monoclonal anti-UBE2E3, clone OTI7E8 (Novus Biologicals, catalog no. NBP2-03819, 1:500), rabbit polyclonal anti-PPP1R2 (Thermo Fisher Scientific, catalog no. PA5-115787, 1:500), rabbit polyclonal anti-NT5C3 (Proteintech, catalog no. 11393-1-AP, 1:500), rabbit polyclonal anti-SZRD1 (Thermo Fisher Scientific, catalog no. A304-742A, 1:1,000), rabbit polyclonal anti-SNRPD1 (Novus Biologicals, catalog no. NBP2-36427, 1:500), rabbit polyclonal anti-DNAJC19 (Thermo Fisher Scientific, catalog no. PA5-98770, 1:1,000), rabbit polyclonal anti-MAPKAP1 (Proteintech, catalog no. 15463-1-AP, 1:500), rabbit monoclonal anti-CD52, clone EPR3153(2) (abcam, catalog no. ab125071, 1:1,000), rabbit polyclonal anti-A2LD1 (Proteintech, catalog no. 23280-1-AP, 1:500), anti-rabbit IgG, horseradish peroxidase (HRP)-linked antibody (Cell Signaling, catalog no. 7074S WB, 1:2,000), anti-mouse IgG, HRP-linked antibody (Cell Signaling catalog no. 7076S, WB 1:2,000). shRNA knockdown and plasmid transfection For transient transfection, cells were transfected with casRx (Addgene catalog no. 109053) gRNA construct and pCMV-TRUB1-dCas13D by Lipofectamine 2000 Transfection Reagent (Invitrogen) according to manufacturer’s protocol, or with siRNA by Lipofectamine RNAiMAX Transfection Reagent (Invitrogen) following commercial protocols. .. For lentivirus production, a lentiviral construct (pLKO-Tet-On for inducible knockdown of pseudouridine synthetases, or green fluorescent protein as a negative control) together with pMD2.G (Addgene catalog no. 12259) and psPAX2 (Addgene catalog no. 12260) were cotransfected into 293TN cells (System Biosciences) as previously described47.



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    Image Search Results


    Summary of antibody dilutions and conditions used in western blot analysis

    Journal: Neural Regeneration Research

    Article Title: MicroRNA-502-3p regulates GABAergic synapse function in hippocampal neurons

    doi: 10.4103/NRR.NRR-D-23-01064

    Figure Lengend Snippet: Summary of antibody dilutions and conditions used in western blot analysis

    Article Snippet: PSN1 rabbit polyclonal , 1:1000 , 16163-1-AP , Proteintech, Rosemont, IL, USA.

    Techniques: Western Blot

    Modulation of AD proteins by miR-502-3p. (A) Immunoblot analysis of amyloid precursor protein (APP), tubulin associated unit (Tau), presenilin-1 (PSN1) and presenilin-2 (PSN2) and -actin protein in hippocampal neurons (HT22) cells transfected with miR-502-3p agomiRs and miR-502-3p antagomiRs. The levels of AD proteins were changed in miR-502-3p agomiR and antagomiR transfected cells. (B) Densitometry analysis of APP, Tau, PSN1 and PSN2 blot shows increased protein levels in miR-502-3p agomiR transfected cells compared with scramble control, while the cells treated with miR-502-3p antagomiR shows a reduced level of all AD proteins compared with agomiRs. For statistical analysis, Student’s t -test was used for analyzing the data between three groups of samples such as scramble control, agomiRs, and antagomiRs. Experiments were performed in triplicates. The P -values < 0.05 were considered statistically significant. AD: Alzheimer’s disease; miR: microRNA.

    Journal: Neural Regeneration Research

    Article Title: MicroRNA-502-3p regulates GABAergic synapse function in hippocampal neurons

    doi: 10.4103/NRR.NRR-D-23-01064

    Figure Lengend Snippet: Modulation of AD proteins by miR-502-3p. (A) Immunoblot analysis of amyloid precursor protein (APP), tubulin associated unit (Tau), presenilin-1 (PSN1) and presenilin-2 (PSN2) and -actin protein in hippocampal neurons (HT22) cells transfected with miR-502-3p agomiRs and miR-502-3p antagomiRs. The levels of AD proteins were changed in miR-502-3p agomiR and antagomiR transfected cells. (B) Densitometry analysis of APP, Tau, PSN1 and PSN2 blot shows increased protein levels in miR-502-3p agomiR transfected cells compared with scramble control, while the cells treated with miR-502-3p antagomiR shows a reduced level of all AD proteins compared with agomiRs. For statistical analysis, Student’s t -test was used for analyzing the data between three groups of samples such as scramble control, agomiRs, and antagomiRs. Experiments were performed in triplicates. The P -values < 0.05 were considered statistically significant. AD: Alzheimer’s disease; miR: microRNA.

    Article Snippet: PSN1 rabbit polyclonal , 1:1000 , 16163-1-AP , Proteintech, Rosemont, IL, USA.

    Techniques: Western Blot, Transfection, Control